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Conserved white rot enzymatic mechanism for wood decay in the Basidiomycota genus Pycnoporus

Shingo Miyauchi 1, 2 Hayat Hage 1 Elodie Drula 1 Laurence Lesage-Meessen 3, 4 Jean-Guy Berrin 3 David Navarro 3, 4 Anne Favel 3, 1 Delphine Chaduli 3, 4 Sacha Grisel 3 Mireille Haon 3 François Piumi 5, 6 Anthony Levasseur 6, 7 Anne Lomascolo 1 Steven Ahrendt 8 Kerrie Barry 8 Kurt Labutti 8 Didier Chevret 9 Chris Daum 8 Jérôme Mariette 10 Christophe Klopp 10 Daniel Cullen 11, 12 Ronald de Vries 13, 14 Allen Gathman 15 Matthieu Hainaut 16, 17 Bernard Henrissat 16, 17 Kristiina Hildén 14 Ursula Kues 18, 19 Walt Lilly 15 Anna Lipzen 20 Miia Mäkelä 14 Angel Martinez 21 Melanie Morel-Rouhier 2 Emmanuelle Morin 2 Jasmyn Pangilinan 22 Arthur Ram 23 Han Wösten 24 Francisco Ruiz-Dueñas 21 Robert Riley 25, 20 Eric Record 1 Igor Grigoriev 26, 27 Marie-Noelle Rosso 1
Abstract : White-rot (WR) fungi are pivotal decomposers of dead organic matter in forest ecosystems and typically use a large array of hydrolytic and oxidative enzymes to deconstruct lignocellulose. However, the extent of lignin and cellulose degradation may vary between species and wood type. Here we combined comparative genomics, transcriptomics and secretome proteomics to identify conserved enzymatic signatures at the onset of wood decaying activity within the Basidiomycota genus Pycnoporus. We observed strong conservation in the genome structures and the repertoires of protein coding genes across the four Pycnoporus species described to date, despite the species having distinct geographic distributions. We further analyzed the early response of P. cinnabarinus, P. coccineus and P. sanguineus to diverse (ligno)-cellulosic substrates. We identified a conserved set of enzymes mobilized by the three species for breaking down cellulose, hemicellulose and pectin. The co-occurrence in the exo-proteomes of H2O2 producing enzymes with H2O2 consuming enzymes was a common feature of the three species, although each enzymatic partner displayed independent transcriptional regulation. Finally, cellobiose dehydrogenase-coding genes were systematically co-regulated with at least one AA9 LPMO gene, indicative of enzymatic synergy in vivo. This study highlights a conserved core white-rot fungal enzymatic mechanism behind the wood decaying process.
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Submitted on : Monday, June 15, 2020 - 2:07:10 PM
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Shingo Miyauchi, Hayat Hage, Elodie Drula, Laurence Lesage-Meessen, Jean-Guy Berrin, et al.. Conserved white rot enzymatic mechanism for wood decay in the Basidiomycota genus Pycnoporus. DNA Research, Oxford University Press (OUP), 2020, ⟨10.1093/dnares/dsaa011⟩. ⟨hal-02868456⟩



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