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Characterisation of the periplasmic methionine sulfoxide reductase (MsrP) from Salmonella Typhimurium

Abstract : The oxidation of free methionine (Met) and Met residues inside proteins leads to the formation of methionine sulfoxide (Met-O). The reduction of Met-O to Met is catalysed by a ubiquitous enzyme family: the methionine sulfoxide reductases (Msr). The importance of Msr systems in bacterial physiology and virulence has been reported in many species. Salmonella Typhimurium, a facultative intracellular pathogen, contains four cytoplasmic Msr. Recently, a periplasmic Msr enzyme (MsrP) has been identified in Escherichia coli. In the present study, the STM14_4072 gene from Salmonella was shown to encode the MsrP protein (StMsrP). We describe the experimental procedure and precautions for the production of this molybdo-enzyme. StMsrP was also demonstrated to reduce free Met-O and to catalyse the complete repair of an oxidized protein. More importantly, this study provides for the first time access to the exhaustive list of the Msr systems of a pathogen, including four cytoplasmic enzymes (MsrA, MsrB, MsrC, BisC) and one periplasmic enzyme (MsrP).
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Submitted on : Tuesday, November 10, 2020 - 10:45:16 AM
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Camille Andrieu, Alexandra Vergnes, Laurent Loiseau, Laurent Aussel, Benjamin Ezraty. Characterisation of the periplasmic methionine sulfoxide reductase (MsrP) from Salmonella Typhimurium. Free Radical Biology and Medicine, Elsevier, 2020, 160, pp.506-512. ⟨10.1016/j.freeradbiomed.2020.06.031⟩. ⟨hal-02995688⟩



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