Alternative Enhancer Usage and Targeted Polycomb Marking Hallmark Promoter Choice during T Cell Differentiation

Muhammad Ahmad Maqbool (1, 2) , Léo Pioger (2) , Amal Zine El Aabidine (2) , Nezih Karasu (3) , Anne Marie Molitor (3) , Lan T M Dao (4) , Guillaume Charbonnier (4) , Francois van Laethem (2) , Romain Fenouil (2) , Frederic Koch (2) , Georges Lacaud (1) , Ivo Gut (5, 6) , Marta Gut (5, 6) , Sebastian Amigorena (7, 8) , Olivier Joffre (9) , Thomas Sexton (3) , Salvatore Spicuglia (4) , Jean-Christophe Andrau (2)

1 University of Manchester [Manchester]
2 IGMM - Institut de Génétique Moléculaire de Montpellier
3 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire
4 TAGC - Theories and Approaches of Genomic Complexity
5 UPF - Universitat Pompeu Fabra [Barcelona]
6 BIST - Barcelona Institute of Science and Technology
7 Institut Curie [Paris]
8 U932 - Immunité et cancer
9 CPTP - Centre de Physiopathologie Toulouse Purpan

Graphical Abstract Highlights d Active T cell p-enhancers are highly dynamic during differentiation d Enhancer diversity might function to select specific isoform expression d Loss of H3K27me3 combined with enhancer gain hallmark T cell identity d Promoter choice is regulated by the PRC2 polycomb complex during differentiation Correspondence [e-mail]. uk (M.A.M.), [e-mail] (J.-C.A.) In Brief Development and activation of T lymphocytes are coordinated by lineage-specific transcriptional programs. Here, Maqbool et al. performed a wide epigenomic and transcriptional analysis of mouse T cell differentiation. These data provide new insights into the role of multiple enhancers and PRC2 in controlling alternative promoter choice during differentiation.

Keywords: T cell enhancerome; enhancer and promoter usage; long-distance enhancer-promoter interactions; enhancer redundancy; CapSTARR-seq

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Immunology; Life Sciences [q-bio]; Biochemistry, Molecular Biology; Genomics [q-bio.GN]

Muhammad Ahmad Maqbool, Léo Pioger, Amal Zine El Aabidine, Nezih Karasu, Anne Marie Molitor, et al.. Alternative Enhancer Usage and Targeted Polycomb Marking Hallmark Promoter Choice during T Cell Differentiation. Cell Reports, 2020, 32, ⟨10.1016/j.celrep.2020.108048⟩. ⟨hal-03015018⟩