Skip to Main content Skip to Navigation
Journal articles

Observation of arenavirus nucleoprotein heptamer assembly

Abstract : Arenaviruses are enveloped viruses containing a segmented, negative and ambisense single‐stranded RNA genome wrapped with a nucleoprotein. The nucleoprotein is the most abundant viral protein in infected cells and plays a critical role in both replication/transcription and virion assembly. The nucleoprotein associates with RNA to form a ribonucleoprotein complex, and this implies self‐assembly while the exact structure of this polymer is not yet known. Here we report a measurement of the full length Mopeia virus NP by negative stain Transmission Electron Microscopy. We observed ribonucleoprotein complex particles with diameter 15 ± 1 nm as well as symmetric circular heptamers of the same diameter, consistent with previous observations.
Document type :
Journal articles
Complete list of metadata

https://hal-amu.archives-ouvertes.fr/hal-03143071
Contributor : François Ferron <>
Submitted on : Tuesday, February 16, 2021 - 3:13:15 PM
Last modification on : Wednesday, March 24, 2021 - 9:21:09 AM
Long-term archiving on: : Monday, May 17, 2021 - 8:10:09 PM

File

2211-5463.13106.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Nicolas Papageorgiou, Afroditi Vaitsopoulou, Awa Diop, Thi van Nguyen, Bruno Canard, et al.. Observation of arenavirus nucleoprotein heptamer assembly. FEBS Open Bio, Wiley Open Access/Elsevier, 2021, ⟨10.1002/2211-5463.13106⟩. ⟨hal-03143071⟩

Share

Metrics

Record views

68

Files downloads

36