Efficiency of a Tetracycline-Adjuvant Combination Against Multidrug Resistant Pseudomonas aeruginosa Tunisian Clinical Isolates

Azza Troudi (1) , Meha Fethi (2) , Mohamed Selim El Asli (3) , Jean Michel Bolla (1) , Naouel Klibi (2) , Jean Michel Brunel (1)

1 MCT - Membranes et cibles thérapeutiques
2 Université de Tunis
3 Hôpital militaire de Tunis

The growing number of multidrug resistant strains in Tunisia has become a serious health concern contributing to high rate of mortality and morbidity. Since current antibiotics are rapidly becoming ineffective, novel strategies to combat resistance are needed. Recently, we demonstrated that combination of a tetracycline antibiotic with various polyaminoisoprenyl adjuvants can sustain the life span and enhance the activity of these drugs against Pseudomonas aeruginosa reference strain (PA01). In the context of our continuing studies, the effective approach of antibiotic-adjuvant was investigated against a large panel of P. aeruginosa Tunisian clinical strains collected from the Military Hospital of Tunis. In this paper, we demonstrated that the combination of a farnesyl spermine compound 3 used at concentrations ranging from 2.5 to 10 µM, in the presence of doxycycline or minocycline leads to a significant decrease of P. aeruginosa antibiotic resistance.

Mots clés: polyaminoisoprenyl derivatives; antibiotic adjuvants; polyamines; Pseudomonas aeruginosa clinical strains; doxycycline; minocycline

Domaines: Sciences du Vivant [q-bio]; Sciences de l'environnement

Azza Troudi, Meha Fethi, Mohamed Selim El Asli, Jean Michel Bolla, Naouel Klibi, et al.. Efficiency of a Tetracycline-Adjuvant Combination Against Multidrug Resistant Pseudomonas aeruginosa Tunisian Clinical Isolates. Antibiotics, 2020, 9 (12), pp.919. ⟨10.3390/antibiotics9120919⟩. ⟨hal-03219853⟩