Skip to Main content Skip to Navigation
Journal articles

RUFY4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages

Abstract : We report here that RUFY4, a newly characterized member of the ‘RUN and FYVE domain-containing’ family of proteins previously associated with autophagy enhancement, is highly expressed in alveolar macrophages (AM). We show that RUFY4 interacts with mitochondria upon stimulation by microbial-associated molecular patterns of AM and dendritic cells. RUFY4 interaction with mitochondria and other organelles is dependent on a previously uncharacterized OmpH domain located immediately upstream of its C-terminal FYVE domain. Further, we demonstrate that rufy4 messenger RNA can be translated from an alternative translation initiation codon, giving rise to a N-terminally truncated form of the molecule lacking most of its RUN domain and with enhanced potential for its interaction with mitochondria. Our observations point towards a role of RUFY4 in selective mitochondria clearance in activated phagocytes.
Document type :
Journal articles
Complete list of metadata

https://hal-amu.archives-ouvertes.fr/hal-03374685
Contributor : Anita Dumenil Connect in order to contact the contributor
Submitted on : Tuesday, October 19, 2021 - 3:54:17 PM
Last modification on : Thursday, October 21, 2021 - 5:18:54 AM

File

RSOS202333.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Jan Valečka, Voahirana Camosseto, David Mcewan, Seigo Terawaki, Zhuangzhuang Liu, et al.. RUFY4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages. Royal Society Open Science, The Royal Society, 2021, 8 (7), pp.202333. ⟨10.1098/rsos.202333⟩. ⟨hal-03374685⟩

Share

Metrics

Record views

36

Files downloads

11