Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, Epiciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Production and properties of non-cytotoxic pyomelanin by laccase and comparison to bacterial and synthetic pigments

Abstract : Abstract Pyomelanin is a polymer of homogentisic acid synthesized by microorganisms. This work aimed to develop a production process and evaluate the quality of the pigment. Three procedures have been elaborated and optimized, (1) an HGA-Mn 2+ chemical autoxidation (Pyo CHEM yield 0.317 g/g substrate), (2) an induced bacterial culture of Halomonas titanicae through the 4-hydroxyphenylacetic acid-1-hydroxylase route (Pyo BACT , 0.55 g/L), and (3) a process using a recombinant laccase extract with the highest level produced (Pyo ENZ , 1.25 g/g substrate) and all the criteria for a large-scale prototype. The chemical structures had been investigated by 13 C solid-state NMR (CP-MAS) and FTIR. C ar –C ar bindings predominated in the three polymers, C ar –O–C ar (ether) linkages being absent, proposing mainly C 3 -C 6 (α-bindings) and C 4 -C 6 (β-bindings) configurations. This work highlighted a biological decarboxylation by the laccase or bacterial oxidase(s), leading to the partly formation of gentisyl alcohol and gentisaldehyde that are integral parts of the polymer. By comparison, Pyo ENZ exhibited an M w of 5,400 Da, was hyperthermostable, non-cytotoxic even after irradiation, scavenged ROS induced by keratinocytes, and had a highly DPPH-antioxidant and Fe 3+ -reducing activity. As a representative pigment of living cells and an available standard, Pyo ENZ might also be useful for applications in extreme conditions and skin protection.
Document type :
Journal articles
Complete list of metadata
Contributor : Patricia BREMOND Connect in order to contact the contributor
Submitted on : Thursday, October 21, 2021 - 1:10:24 PM
Last modification on : Friday, April 1, 2022 - 3:46:10 AM
Long-term archiving on: : Saturday, January 22, 2022 - 6:49:49 PM


Publication funded by an institution


Distributed under a Creative Commons Attribution 4.0 International License



Faustine Lorquin, Fabio Ziarelli, Agnès Amouric, Carole Di Giorgio, Maxime Robin, et al.. Production and properties of non-cytotoxic pyomelanin by laccase and comparison to bacterial and synthetic pigments. Scientific Reports, Nature Publishing Group, 2021, 11 (1), pp.8538. ⟨10.1038/s41598-021-87328-2⟩. ⟨hal-03375950⟩



Record views


Files downloads