Adapter Protein SH2-B Stimulates Actin-Based Motility of Listeria monocytogenes in a Vasodilator-Stimulated Phosphoprotein (VASP)-Dependent Fashion

Abstract : SH2-Bbeta (Src homology 2 B) is an adapter protein that is required for maximal growth hormone-dependent actin reorganization in membrane ruffling and cell motility. Here we show that SH2-Bbeta is also required for maximal actin-based motility of Listeria monocytogenes. SH2-Bbeta localizes to Listeria-induced actin tails and increases the rate of bacterial propulsion in infected cells and in cell extracts. Furthermore, Listeria motility is decreased in mouse embryo fibroblasts from SH2-B -/- mice. Both recruitment of SH2-Bbeta to Listeria and SH2-Bbeta stimulation of actin-based propulsion require the vasodilator-stimulated phosphoprotein (VASP), which binds ActA at the surfaces of Listeria cells and enhances bacterial actin-based motility. SH2-Bbeta enhances actin-based movement of ActA-coated beads in a biomimetic actin-based motility assay, provided that VASP is present. In vitro binding assays show that SH2-Bbeta binds ActA but not VASP; however, binding to ActA is greater in the presence of VASP. Because VASP also plays an essential regulatory role in actin-based processes in eukaryotic cells, the present results provide mechanistic insight into the functions of both SH2-Bbeta and VASP in motility and also increase our understanding of the fundamental mechanism by which Listeria spreads.
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-01960836
Contributor : Emmanuèle Helfer <>
Submitted on : Thursday, January 10, 2019 - 2:56:22 PM
Last modification on : Thursday, February 7, 2019 - 4:21:26 PM
Long-term archiving on : Thursday, April 11, 2019 - 2:55:05 PM

File

IAI2007_Diakonova&al.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Maria Diakonova, Emmanuèle Helfer, Stéphanie Seveau, Joel A. Swanson, Christine Kocks, et al.. Adapter Protein SH2-B Stimulates Actin-Based Motility of Listeria monocytogenes in a Vasodilator-Stimulated Phosphoprotein (VASP)-Dependent Fashion. Infection and Immunity, American Society for Microbiology, 2007, 75 (7), pp.3581-3593. ⟨10.1128/iai.00214-07⟩. ⟨hal-01960836⟩

Share

Metrics

Record views

55

Files downloads

65