Early-stage knee OA induced by MIA and MMT compared in the murine model via histological and topographical approaches

Gaetan Aüllo-Rasser (1, 2) , Erick Dousset (2) , Sandrine Roffino (2, 1) , Hassan Zahouani (3) , Roger Lecurieux-Clerville , Jean-Noël Argenson (2, 1) , Patrick Chabrand (2, 1)

1 IML - Institut du Mouvement et de l’appareil Locomoteur [Hôpital Sainte-Marguerite - APHM]
2 ISM - Institut des Sciences du Mouvement Etienne Jules Marey
3 LTDS - Laboratoire de Tribologie et Dynamique des Systèmes

Osteoarthritis (OA) is a common degenerative disease whose early management includes promising mechanical treatments. New treatments are initially validated using an animal model in which OA is induced. The MMT (mechanical induction) and MIA (chemical induction) models of OA induction are widespread, but their use to generate early OA is poorly documented. We analyzed and compared early-stage knee OA-induction via these two methods in 16 rats divided into two groups. After 4 weeks of induction, the knees were sampled and studied using both histology (Toluidine Blue and Sirius Red) and surface topology, an innovative technique for characterizing osteoarthritic cartilage. The Mankin-modified score confirms that the two OA-induction models evolved at the same speed. At this early stage, the two models can be differentiated morphologically, although no significant differences were revealed by either cellularity or birefringence analysis. However, the topological analysis generated two forms of quantitative data, the deformation ratio and the cohesion index, that differentiated between the two groups. Thus, the early-stage OA induced by these two models is revealed to differ. The patterns of cartilage damage induced point to MMT as the better choice to assess mechanical approaches to clinical OA treatment.

Domaines: Rhumatologie et système ostéo-articulaire; Biomécanique [physics.med-ph]

Gaetan Aüllo-Rasser, Erick Dousset, Sandrine Roffino, Hassan Zahouani, Roger Lecurieux-Clerville, et al.. Early-stage knee OA induced by MIA and MMT compared in the murine model via histological and topographical approaches. Scientific Reports, 2020, 10, pp.15430. ⟨10.1038/s41598-020-72350-7⟩. ⟨hal-03035605⟩