Skip to Main content Skip to Navigation
Journal articles

Performance of MALDI-TOF Mass Spectrometry to Determine the Sex of Mosquitoes and Identify Specific Colonies from French Polynesia

Fatou Kiné Fall 1 Maureen Laroche 2 Hervé Bossin 3 Didier Musso 2 Philippe Parola 2
1 Campus International UCAD-IRD [Dakar, Senegal]
2 VITROME - Vecteurs - Infections tropicales et méditerranéennes
3 ILM - Institut Louis Malardé [Papeete]
Abstract : ABSTRACT Mosquitoes are the main arthropod vectors of human pathogens. The current methods for mosquito identification include morphological and molecular methods. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), now routinely used for bacterial identification, has recently emerged in the field of entomology. The aim of this study was to use MALDI-TOF MS to identify mosquito colonies from French Polynesia. Five hundred specimens from French Polynesia belonging to three species, Aedes aegypti , Aedes polynesiensis , and Culex quinquefasciatus , were included in the study. Testing the legs of these mosquitoes by MALDI-TOF MS revealed a 100% correct identification of all specimens at the species level. The MALDI-TOF MS profiles obtained allowed differentiation of male from female mosquitoes and the specific identification of female mosquito colonies of the same species but different geographic origin.
Document type :
Journal articles
Complete list of metadata
https://hal-amu.archives-ouvertes.fr/hal-03304666
Contributor : Isabelle Combe <>
Submitted on : Wednesday, July 28, 2021 - 3:45:02 PM
Last modification on : Thursday, July 29, 2021 - 3:07:58 AM

Identifiers

Collections

UNIV-AMU

Citation

Fatou Kiné Fall, Maureen Laroche, Hervé Bossin, Didier Musso, Philippe Parola. Performance of MALDI-TOF Mass Spectrometry to Determine the Sex of Mosquitoes and Identify Specific Colonies from French Polynesia. American Journal of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene, 2021, 104 (5), pp.1907-1916. ⟨10.4269/ajtmh.20-0031⟩. ⟨hal-03304666⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

12