Performance of MALDI-TOF Mass Spectrometry to Determine the Sex of Mosquitoes and Identify Specific Colonies from French Polynesia - Archive ouverte HAL Access content directly
Journal Articles American Journal of Tropical Medicine and Hygiene Year : 2021

Performance of MALDI-TOF Mass Spectrometry to Determine the Sex of Mosquitoes and Identify Specific Colonies from French Polynesia

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Abstract

ABSTRACT Mosquitoes are the main arthropod vectors of human pathogens. The current methods for mosquito identification include morphological and molecular methods. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), now routinely used for bacterial identification, has recently emerged in the field of entomology. The aim of this study was to use MALDI-TOF MS to identify mosquito colonies from French Polynesia. Five hundred specimens from French Polynesia belonging to three species, Aedes aegypti , Aedes polynesiensis , and Culex quinquefasciatus , were included in the study. Testing the legs of these mosquitoes by MALDI-TOF MS revealed a 100% correct identification of all specimens at the species level. The MALDI-TOF MS profiles obtained allowed differentiation of male from female mosquitoes and the specific identification of female mosquito colonies of the same species but different geographic origin.

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hal-03304666 , version 1 (28-07-2021)

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Fatou Kiné Fall, Maureen Laroche, Hervé Bossin, Didier Musso, Philippe Parola. Performance of MALDI-TOF Mass Spectrometry to Determine the Sex of Mosquitoes and Identify Specific Colonies from French Polynesia. American Journal of Tropical Medicine and Hygiene, 2021, 104 (5), pp.1907-1916. ⟨10.4269/ajtmh.20-0031⟩. ⟨hal-03304666⟩

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