Journal articles
Performance of MALDI-TOF Mass Spectrometry to Determine the Sex of Mosquitoes and Identify Specific Colonies from French Polynesia
Abstract : ABSTRACT Mosquitoes are the main arthropod vectors of human pathogens. The current methods for mosquito identification include morphological and molecular methods. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), now routinely used for bacterial identification, has recently emerged in the field of entomology. The aim of this study was to use MALDI-TOF MS to identify mosquito colonies from French Polynesia. Five hundred specimens from French Polynesia belonging to three species, Aedes aegypti , Aedes polynesiensis , and Culex quinquefasciatus , were included in the study. Testing the legs of these mosquitoes by MALDI-TOF MS revealed a 100% correct identification of all specimens at the species level. The MALDI-TOF MS profiles obtained allowed differentiation of male from female mosquitoes and the specific identification of female mosquito colonies of the same species but different geographic origin.
https://hal-amu.archives-ouvertes.fr/hal-03304666
Contributor : Isabelle COMBE Connect in order to contact the contributor
Submitted on : Wednesday, July 28, 2021 - 3:45:02 PM
Last modification on : Sunday, June 26, 2022 - 10:18:39 AM
Contributor : Isabelle COMBE Connect in order to contact the contributor
Submitted on : Wednesday, July 28, 2021 - 3:45:02 PM
Last modification on : Sunday, June 26, 2022 - 10:18:39 AM
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