Journal articles
Detection of Rickettsia raoultii in Dermacentor reticulatus and Haemaphysalis inermis ticks in Slovakia
Abstract : Ticks are vector arthropods responsible for the transmission of several pathogenic agents that affect both human and animal health worldwide. In this study our objective was to analyse, using molecular tools, the bacterial community of Dermacentor reticulatus and Haemaphysalis inermis ticks collected in south-eastern Slovakia. Using real-time PCR, we identified the presence of Rickettsia spp. DNA at levels of 14/59 (23.72 %) and 29/173 (16.76 %) in D. reticulatus and H. inermis , respectively. In addition, using standard PCR and sequencing, we identified the presence of Rickettsia raoultii DNA in 13 ticks belonging to the two investigated species. Rickettsia raoultii blast results revealed an average identification percentage of 99.62 %. Following the results of this molecular study there is a possibility that D. reticulatus and H. inermis play a potential role in the transmission of R. raoultii . To prove the possibility of validity of this hypothesis, we suggest performing experimental models in future studies. Our results can serve as preliminary data for future transmission models.
https://hal-amu.archives-ouvertes.fr/hal-03334365
Contributor : Isabelle Combe <>
Submitted on : Monday, September 6, 2021 - 9:05:45 AM
Last modification on : Sunday, September 12, 2021 - 9:05:36 PM
Contributor : Isabelle Combe <>
Submitted on : Monday, September 6, 2021 - 9:05:45 AM
Last modification on : Sunday, September 12, 2021 - 9:05:36 PM
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