Immunocytochemical Detection of ALK and ROS1 Rearrangements in Lung Cancer Cytological Samples

Diane Frankel (1, 2) , Elise Kaspi (1, 2) , Patrice Roll (1, 2)

1 Laboratoire de Biologie Cellulaire [Hôpital de la Timone - APHM]
2 MMG - Marseille medical genetics - Centre de génétique médicale de Marseille

The detection of molecular alterations such as ROS1 and ALK rearrangements is performed as part of the diagnosis of advanced-stage lung adenocarcinoma. These alterations allow the treatments with tyrosine kinase inhibitors. Cytological samples are very useful as up to 40% patients are diagnosed with this type of sample. Here we describe the immunocytochemistry technique usable to reveal the overexpression of ALK or ROS1 tyrosine kinase receptors secondary to ALK and ROS1 rearrangements, respectively.

Keywords: Immunocytochemistry; ALK; ROS1; Lung cancer; Adenocarcinoma; Cytology

Domains: Life Sciences [q-bio]

Diane Frankel, Elise Kaspi, Patrice Roll. Immunocytochemical Detection of ALK and ROS1 Rearrangements in Lung Cancer Cytological Samples. Lung Cancer, 2279, Springer US; Springer US, pp.157-164, 2021, Methods in Molecular Biology, ⟨10.1007/978-1-0716-1278-1_12⟩. ⟨hal-03508593⟩