Limosilactobacillus caccae sp. nov., a new bacterial species isolated from the human gut microbiota

Cheikh Ibrahima Lo (1, 2) , Niokhor Dione (3, 2) , Aminata Mbaye (3, 2) , Patricia Fernández-Mellado Gómez (3, 2) , Issa Isaac Ngom (3, 2) , Camille Valles (3, 2) , Stéphane Alibar (3, 2) , Jean-Christophe Lagier (3, 2) , Florence Fenollar (1, 2) , Pierre-Edouard Fournier (1, 2) , Didier Raoult (2, 3) , Seydina Diene (2, 3)

1 VITROME - Vecteurs - Infections tropicales et méditerranéennes
2 IHU Marseille - Institut Hospitalier Universitaire Méditerranée Infection
3 MEPHI - Microbes évolution phylogénie et infections

ABSTRACT Strain Marseille-P3519T isolated from the fecal flora of a 25-year-old healthy French woman was a Gram-positive anaerobic bacterium, non-motile and non-spore forming. The 16S rRNA gene sequence of Marseille-P3519 showed 97.73% of sequence similarity with Limosilactobacillus reuteri DSM 20016, the closest species, phylogenetically. Furthermore, the average nucleotide identity of strain Marseille-3519 with its closest related species was 75.8% that was very below the recommended threshold (>95–96%). Its genome had 2 237 367 bp with 45.42 mol% of G + C content. Major fatty acids were C16:0 (50.8%), C18:1n9 (18.0%), C18:2n6 (9.8%) and C19:1n9 (8.9%). It was catalase negative and fermented glycerol, glucose, fructose, D-maltose, lactose and mannose. These findings support that strain Marseille-P3519 ( = CSURP3519 = CECT 30110) is a new member of the genus Limosilactobacillus for which the name Limosilactobacillus caccae sp. nov., is proposed.

Domaines: Bactériologie; Virologie; Parasitologie; Cardiologie et système cardiovasculaire; Maladies émergentes; Maladies infectieuses

Cheikh Ibrahima Lo, Niokhor Dione, Aminata Mbaye, Patricia Fernández-Mellado Gómez, Issa Isaac Ngom, et al.. Limosilactobacillus caccae sp. nov., a new bacterial species isolated from the human gut microbiota. FEMS Microbiology Letters, 2021, 368 (18), ⟨10.1093/femsle/fnab128⟩. ⟨hal-03636870⟩