Quantification of Antifibrillarin (anti-U3 RNP) Antibodies: A New Insight for Patients with Systemic Sclerosis - Archive ouverte HAL Access content directly
Journal Articles Diagnostics Year : 2021

Quantification of Antifibrillarin (anti-U3 RNP) Antibodies: A New Insight for Patients with Systemic Sclerosis

(1, 2) , (3) , (4, 5) , (1, 3) , (1) , (6, 7, 8) , (7) , (9, 10, 11) , (9, 10, 11, 12) , (13) , (13) , (14) , (14) , (4) , (1, 3) , (2, 1) , (1, 3) , (1, 2)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
Daniel Bertin
Sylvain Dubucquoi
  • Function : Author

Abstract

Background: The detection of additional autoantibodies is of great concern in systemic sclerosis (SSc) when those included in the ACR/EULAR classification are negative. In this context, the interest of antifibrillarin (anti-U3RNP) autoantibodies (AFAs) in the routine evaluation of SSc remains unclear. We aimed to assess the relevance of AFAs and their clinical association in SSc patients. Methods: In a multicenter observational retrospective study, we collected immunological and clinical data associated with AFA positivity in SSc (n = 42) and non-SSc patients (n = 13). Patients with SSc negative for AFAs (n = 83) were considered as a control group. AFAs were detected by indirect immunofluorescence (IIF) using HEp-2 cells, EliA or immunoblot techniques. Results: We confirmed a typical nuclear IIF pattern and showed that AFAs are mostly exclusive towards SSc conventional autoantibodies. Although also observed in non-SSc patients, high levels of AFAs with the ELiA technique allowed the diagnosis of SSc. Compared to AFA-negative SSc patients, AFA-positive SSc patients more frequently exhibited visceral involvements. They more frequently suffered from the diffuse cutaneous form and had a higher global severity of the disease. Conclusions: We demonstrate the usefulness of quantifying AFAs in the immunological exploration of SSc, especially when patients are seronegative for SSc conventional autoantibodies and display a typical IIF pattern. AFAs might constitute an interesting marker of SSc severity.

Dates and versions

hal-03653470 , version 1 (27-04-2022)

Identifiers

Cite

Audrey Benyamine, Daniel Bertin, Noémie Resseguier, Xavier Heim, Julien Bermudez, et al.. Quantification of Antifibrillarin (anti-U3 RNP) Antibodies: A New Insight for Patients with Systemic Sclerosis. Diagnostics, 2021, 11 (6), pp.1064. ⟨10.3390/diagnostics11061064⟩. ⟨hal-03653470⟩
12 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More