Flow cytometry evaluation of infection-related biomarkers in febrile subjects in the emergency department

Penelope Bourgoin (1) , Guillaume Lediagon (2) , Isabelle Arnoux (2) , Denis Bernot (2) , Pierre-Emmanuel Morange (1, 3) , Pierre Michelet (2) , Fabrice Malergue (4) , Thibaut Markarian (2)

1 C2VN - Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research
2 TIMONE - Hôpital de la Timone [CHU - APHM]
3 Laboratoire d'hématologie biologique [Hôpital de la Timone - Hôpital Nord - APHM]
4 Immunotech-Beckman Coulter

Aim: In an Emergency Department (ED), the etiological identification of infected subjects is essential. 13 infection-related biomarkers were assessed using a new flow cytometry procedure. Materials & methods: If subjects presented with febrile symptoms at the ED, 13 biomarkers' levels, including CD64 on neutrophils (nCD64) and CD169 on monocytes (mCD169), were tested and compared with clinical records. Results: Among 50 subjects, 78% had bacterial infections and 8% had viral infections. nCD64 showed 82% sensitivity and 91% specificity for identifying subjects with bacterial infections. mCD169, HLA-ABC ratio and HLA-DR on monocytes had high values in subjects with viral infections. Conclusion: Biomarkers showed promising performances to improve the ED's infectious stratification.

Mots clés: bacterial infection; EARLY-DIAGNOSIS; BACTERIAL-INFECTION; C-REACTIVE PROTEIN; viral infection; flow cytometry; fever; emergency department; EXAMPLE; DIFFERENTIATE; PROCALCITONIN; CD64; MARKERS; ACCURACY; EXPRESSION

Domaines: Sciences du Vivant [q-bio]; Médecine humaine et pathologie

Penelope Bourgoin, Guillaume Lediagon, Isabelle Arnoux, Denis Bernot, Pierre-Emmanuel Morange, et al.. Flow cytometry evaluation of infection-related biomarkers in febrile subjects in the emergency department. Future Microbiology, 2020, 15 (3), pp.189-201. ⟨10.2217/fmb-2019-0256⟩. ⟨hal-03163533⟩