Vitamin A Deficiency during the Perinatal Period and First Weeks of Life Modifies Vitamin A and Lipid Postprandial Metabolism in Both Female and Male Young Rats

Tiffany Antoine (1) , Patrick Borel (1) , Roland Govers (1) , Laure Meiller (2) , Philippe Guichard (1) , Charlotte Halimi (1) , Teresa Gonzalez (1) , Marion Nowicki (1) , Valérie Sauvinet (2) , Michel Grino (1) , Emmanuelle Reboul (1)

1 C2VN - Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research
2 CRNH-RA - Centre de Recherche en Nutrition Humaine Rhône-Alpes

Scope The effect of vitamin A deficiency on vitamin A and lipid postprandial metabolism in young rats is addressed, considering the effect of sex. Methods and Results Sprague-Dawley rats are fed either 400 UI.kg(-1) vitamin A diet (vitamin A-deficient (VAD) diet) or 2300 UI.kg(-1) vitamin A (control diet), before being mated. Mothers receive the same VAD or control diet during gestation and lactation. Offspring receive the same diet than mothers until 8 weeks of age. VAD diet-fed female and male offspring display a severe vitamin A deficiency with no body weight or glucose tolerance defects. Fasting plasma triglyceride concentrations are decreased in VAD diet-fed animals compared to controls (p < 0.05). Retinyl ester postprandial responses after vitamin A gavage, expressed as area under the curves, are not different in VAD diet-fed and control animals, although retinyl ester postprandial peak is significantly delayed (p < 0.05) in VAD diet-fed rats. Lipids also accumulate in the distal part of the intestine after gavage and [1-C-13]-oleate postprandial response is decreased in VAD diet-fed males. Conclusion Vitamin A deficiency modulates both vitamin A absorption rate and lipid postprandial metabolism, which can partly explain the altered fasting lipid status observed in VAD diet-fed offspring.

Mots clés: [1-C-13]-oleate; adipose tissue; bioavailability; intestine; liver; retinol; retinyl esters

Domaines: Sciences du Vivant [q-bio]

Tiffany Antoine, Patrick Borel, Roland Govers, Laure Meiller, Philippe Guichard, et al.. Vitamin A Deficiency during the Perinatal Period and First Weeks of Life Modifies Vitamin A and Lipid Postprandial Metabolism in Both Female and Male Young Rats. Molecular Nutrition and Food Research, 2021, 65 (21), pp.2100451. ⟨10.1002/mnfr.202100451⟩. ⟨hal-03612097⟩